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trypsin edta solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher trypsin edta solution
    Trypsin Edta Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypsin+edta+solution/Trypsin/pmc13101689-168-9-11
    Average 99 stars, based on 1 article reviews
    trypsin edta solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Modification:

    Article Title: An AI-assisted designed supramolecularly engineered nanoplatform reverses pigmentation by triggering an ineffective compensatory melanin production program
    Article Snippet: .. Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin solution, and trypsin-EDTA solution were purchased from Gibco (Grand Island, NY, USA). .. Antibodies against DCT, MITF, and TYR were purchased from Merck (Darmstadt, Germany).

    Article Title: An injectable thermosensitive PLGA–PEG–PLGA hydrogel integrated with coordination-driven self-assembled MTX–Mn nanoparticles for enhanced melanoma therapy via mitochondrial dysfunction
    Article Snippet: .. The essential reagents for cell culture included 1% penicillin–streptomycin solution (PS), fetal bovine serum (FBS), trypsin–EDTA solution, and Dulbecco's Modified Eagle Medium (DMEM), all purchased from Gibco (New York, USA). .. The mouse melanoma cell line B16F10 was obtained from Servicebio Biotechnology Co., Ltd (Wuhan, China).

    Saline:

    Article Title: From N-of-1 to versatility in propionic acidemia: Antisense oligonucleotide-mediated skipping of a constitutive PCCA pseudoexon
    Article Snippet: .. Forty-eight hours post-transfection, the fibroblasts were detached using 0.25% trypsin/EDTA solution (Thermo Fisher Scientific), washed once with phosphate-buffered saline, centrifuged to obtain a cell pellet of 1 × 10 6 cells, and flash-frozen in liquid nitrogen. ..

    Article Title: LDHA protects vascular endothelial cells from oxidative stress-induced mitochondrial damage via HIF-1α activation and glycolytic reprogramming
    Article Snippet: The nuclear and cytoplasmic proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Inc.) following the manufacturer's instructions. .. For flow cytometry, VECs were detached utilizing a trypsin-EDTA solution (Gibco; Thermo Fisher Scientific, Inc.), followed by a wash with phosphate-buffered saline (PBS). .. The cells (1×10 6 ) were subsequently stained with an Annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit (Beyotime Institute of Biotechnology), in accordance with the manufacturer's instructions.

    Flow Cytometry:

    Article Title: LDHA protects vascular endothelial cells from oxidative stress-induced mitochondrial damage via HIF-1α activation and glycolytic reprogramming
    Article Snippet: The nuclear and cytoplasmic proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Inc.) following the manufacturer's instructions. .. For flow cytometry, VECs were detached utilizing a trypsin-EDTA solution (Gibco; Thermo Fisher Scientific, Inc.), followed by a wash with phosphate-buffered saline (PBS). .. The cells (1×10 6 ) were subsequently stained with an Annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit (Beyotime Institute of Biotechnology), in accordance with the manufacturer's instructions.

    Cell Culture:

    Article Title: An injectable thermosensitive PLGA–PEG–PLGA hydrogel integrated with coordination-driven self-assembled MTX–Mn nanoparticles for enhanced melanoma therapy via mitochondrial dysfunction
    Article Snippet: .. The essential reagents for cell culture included 1% penicillin–streptomycin solution (PS), fetal bovine serum (FBS), trypsin–EDTA solution, and Dulbecco's Modified Eagle Medium (DMEM), all purchased from Gibco (New York, USA). .. The mouse melanoma cell line B16F10 was obtained from Servicebio Biotechnology Co., Ltd (Wuhan, China).



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